16-plex tmt reagent Search Results


16 plex tandem mass tag tmt reagents  (Thermo Fisher)
99
Thermo Fisher 16 plex tandem mass tag tmt reagents
16 Plex Tandem Mass Tag Tmt Reagents, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/16 plex tandem mass tag tmt reagents/product/Thermo Fisher
Average 99 stars, based on 1 article reviews
16 plex tandem mass tag tmt reagents - by Bioz Stars, 2026-02
99/100 stars
  Buy from Supplier
tmt pro 16-plex  (Thermo Fisher)
90
Thermo Fisher tmt pro 16-plex
Tmt Pro 16 Plex, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/tmt pro 16-plex/product/Thermo Fisher
Average 90 stars, based on 1 article reviews
tmt pro 16-plex - by Bioz Stars, 2026-02
90/100 stars
  Buy from Supplier
13 plex tmt label reagent kit  (Thermo Fisher)
90
Thermo Fisher 13 plex tmt label reagent kit
13 Plex Tmt Label Reagent Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/13 plex tmt label reagent kit/product/Thermo Fisher
Average 90 stars, based on 1 article reviews
13 plex tmt label reagent kit - by Bioz Stars, 2026-02
90/100 stars
  Buy from Supplier
tandem mass tag (tmt) 16plex reagents  (Thermo Fisher)
90
Thermo Fisher tandem mass tag (tmt) 16plex reagents
Tandem Mass Tag (Tmt) 16plex Reagents, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/tandem mass tag (tmt) 16plex reagents/product/Thermo Fisher
Average 90 stars, based on 1 article reviews
tandem mass tag (tmt) 16plex reagents - by Bioz Stars, 2026-02
90/100 stars
  Buy from Supplier
12plex tmt label reagent kit  (Thermo Fisher)
90
Thermo Fisher 12plex tmt label reagent kit
12plex Tmt Label Reagent Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/12plex tmt label reagent kit/product/Thermo Fisher
Average 90 stars, based on 1 article reviews
12plex tmt label reagent kit - by Bioz Stars, 2026-02
90/100 stars
  Buy from Supplier
16-plex tandem mass tag (tmt) reagents  (Thermo Fisher)
90
Thermo Fisher 16-plex tandem mass tag (tmt) reagents
E2 knockdown in HEK293T cells was obtained via siRNAs for individual E2s or for groups of related E2s with high sequence homology (e.g. UBE2D1/2/3), which were targeted together by pooling E2-specific siRNAs. E2 siRNAs reduce (green) the mRNA ( a ) and protein ( b ) levels of the targeted E2s (boxed) specifically, i.e. without affecting other E2s, compared to non-targeting (NT) siRNAs. Significant ( P < 0.05; unpaired two-tailed t test) transcriptional changes are highlighted in bold red (downregulated) and blue fonts (upregulated). The extent of downregulation is displayed in green shades whereas non-significant changes are shown in gray. c Deep-coverage TMT mass spectrometry identifies the proteome subsets that are regulated by E2 RNAi. Compared to NT siRNAs, E2 RNAi leads to significant protein upregulation (blue) and downregulation (red) ( P < 0.05; Log 2 FC > 0.2 and <–0.2) that do not arise from corresponding changes in mRNA levels, defined by RNA-seq. This data represents 6 sets of <t>16-plex</t> TMTs of E2 siRNAs ( n = 3 biological replicates/group), each with its own control NT siRNAs ( n = 4 biological replicates). On average, each TMT set quantified 10700 proteins: 5132 of these (mapping to 4676 DAVID IDs) were modulated by ≥1 E2s. d JUMPptm analyses identify linkage-specific ubiquitin modifications modulated by E2s. In particular, the knockdown of many E2s reduces K48-linked ubiquitination whereas this increases upon heat shock. Other linkage-specific modifications (K6, K11, K27, K29, K33, and K63) are affected by fewer E2s. Significant downregulation ( P < 0.05) of these modifications by E2 RNAi is displayed in bold fonts and shades of red whereas non-significant changes ( P > 0.05) are shown in gray. Supplementary Fig. reports the precise P values (one-way ANOVA). e Proteins that are regulated by E2 RNAi without significant mRNA changes are reported on the y -axis whereas the number of E2s regulating each protein subset is reported on the x -axis. Several GO categories are enriched among protein sets that are modulated by 5–9 E2s ( f ), 2–4 E2s ( g ), and by single E2s ( h ). Source data are provided in the Source data file.
16 Plex Tandem Mass Tag (Tmt) Reagents, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/16-plex tandem mass tag (tmt) reagents/product/Thermo Fisher
Average 90 stars, based on 1 article reviews
16-plex tandem mass tag (tmt) reagents - by Bioz Stars, 2026-02
90/100 stars
  Buy from Supplier
tmt 16-plex isobaric label reagents  (Thermo Fisher)
90
Thermo Fisher tmt 16-plex isobaric label reagents
E2 knockdown in HEK293T cells was obtained via siRNAs for individual E2s or for groups of related E2s with high sequence homology (e.g. UBE2D1/2/3), which were targeted together by pooling E2-specific siRNAs. E2 siRNAs reduce (green) the mRNA ( a ) and protein ( b ) levels of the targeted E2s (boxed) specifically, i.e. without affecting other E2s, compared to non-targeting (NT) siRNAs. Significant ( P < 0.05; unpaired two-tailed t test) transcriptional changes are highlighted in bold red (downregulated) and blue fonts (upregulated). The extent of downregulation is displayed in green shades whereas non-significant changes are shown in gray. c Deep-coverage TMT mass spectrometry identifies the proteome subsets that are regulated by E2 RNAi. Compared to NT siRNAs, E2 RNAi leads to significant protein upregulation (blue) and downregulation (red) ( P < 0.05; Log 2 FC > 0.2 and <–0.2) that do not arise from corresponding changes in mRNA levels, defined by RNA-seq. This data represents 6 sets of <t>16-plex</t> TMTs of E2 siRNAs ( n = 3 biological replicates/group), each with its own control NT siRNAs ( n = 4 biological replicates). On average, each TMT set quantified 10700 proteins: 5132 of these (mapping to 4676 DAVID IDs) were modulated by ≥1 E2s. d JUMPptm analyses identify linkage-specific ubiquitin modifications modulated by E2s. In particular, the knockdown of many E2s reduces K48-linked ubiquitination whereas this increases upon heat shock. Other linkage-specific modifications (K6, K11, K27, K29, K33, and K63) are affected by fewer E2s. Significant downregulation ( P < 0.05) of these modifications by E2 RNAi is displayed in bold fonts and shades of red whereas non-significant changes ( P > 0.05) are shown in gray. Supplementary Fig. reports the precise P values (one-way ANOVA). e Proteins that are regulated by E2 RNAi without significant mRNA changes are reported on the y -axis whereas the number of E2s regulating each protein subset is reported on the x -axis. Several GO categories are enriched among protein sets that are modulated by 5–9 E2s ( f ), 2–4 E2s ( g ), and by single E2s ( h ). Source data are provided in the Source data file.
Tmt 16 Plex Isobaric Label Reagents, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/tmt 16-plex isobaric label reagents/product/Thermo Fisher
Average 90 stars, based on 1 article reviews
tmt 16-plex isobaric label reagents - by Bioz Stars, 2026-02
90/100 stars
  Buy from Supplier
16-plex tandem mass tag (tmt) pro reagents  (Thermo Fisher)
90
Thermo Fisher 16-plex tandem mass tag (tmt) pro reagents
E2 knockdown in HEK293T cells was obtained via siRNAs for individual E2s or for groups of related E2s with high sequence homology (e.g. UBE2D1/2/3), which were targeted together by pooling E2-specific siRNAs. E2 siRNAs reduce (green) the mRNA ( a ) and protein ( b ) levels of the targeted E2s (boxed) specifically, i.e. without affecting other E2s, compared to non-targeting (NT) siRNAs. Significant ( P < 0.05; unpaired two-tailed t test) transcriptional changes are highlighted in bold red (downregulated) and blue fonts (upregulated). The extent of downregulation is displayed in green shades whereas non-significant changes are shown in gray. c Deep-coverage TMT mass spectrometry identifies the proteome subsets that are regulated by E2 RNAi. Compared to NT siRNAs, E2 RNAi leads to significant protein upregulation (blue) and downregulation (red) ( P < 0.05; Log 2 FC > 0.2 and <–0.2) that do not arise from corresponding changes in mRNA levels, defined by RNA-seq. This data represents 6 sets of <t>16-plex</t> TMTs of E2 siRNAs ( n = 3 biological replicates/group), each with its own control NT siRNAs ( n = 4 biological replicates). On average, each TMT set quantified 10700 proteins: 5132 of these (mapping to 4676 DAVID IDs) were modulated by ≥1 E2s. d JUMPptm analyses identify linkage-specific ubiquitin modifications modulated by E2s. In particular, the knockdown of many E2s reduces K48-linked ubiquitination whereas this increases upon heat shock. Other linkage-specific modifications (K6, K11, K27, K29, K33, and K63) are affected by fewer E2s. Significant downregulation ( P < 0.05) of these modifications by E2 RNAi is displayed in bold fonts and shades of red whereas non-significant changes ( P > 0.05) are shown in gray. Supplementary Fig. reports the precise P values (one-way ANOVA). e Proteins that are regulated by E2 RNAi without significant mRNA changes are reported on the y -axis whereas the number of E2s regulating each protein subset is reported on the x -axis. Several GO categories are enriched among protein sets that are modulated by 5–9 E2s ( f ), 2–4 E2s ( g ), and by single E2s ( h ). Source data are provided in the Source data file.
16 Plex Tandem Mass Tag (Tmt) Pro Reagents, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/16-plex tandem mass tag (tmt) pro reagents/product/Thermo Fisher
Average 90 stars, based on 1 article reviews
16-plex tandem mass tag (tmt) pro reagents - by Bioz Stars, 2026-02
90/100 stars
  Buy from Supplier
tmt 16plex reagents a44522  (Thermo Fisher)
90
Thermo Fisher tmt 16plex reagents a44522
E2 knockdown in HEK293T cells was obtained via siRNAs for individual E2s or for groups of related E2s with high sequence homology (e.g. UBE2D1/2/3), which were targeted together by pooling E2-specific siRNAs. E2 siRNAs reduce (green) the mRNA ( a ) and protein ( b ) levels of the targeted E2s (boxed) specifically, i.e. without affecting other E2s, compared to non-targeting (NT) siRNAs. Significant ( P < 0.05; unpaired two-tailed t test) transcriptional changes are highlighted in bold red (downregulated) and blue fonts (upregulated). The extent of downregulation is displayed in green shades whereas non-significant changes are shown in gray. c Deep-coverage TMT mass spectrometry identifies the proteome subsets that are regulated by E2 RNAi. Compared to NT siRNAs, E2 RNAi leads to significant protein upregulation (blue) and downregulation (red) ( P < 0.05; Log 2 FC > 0.2 and <–0.2) that do not arise from corresponding changes in mRNA levels, defined by RNA-seq. This data represents 6 sets of <t>16-plex</t> TMTs of E2 siRNAs ( n = 3 biological replicates/group), each with its own control NT siRNAs ( n = 4 biological replicates). On average, each TMT set quantified 10700 proteins: 5132 of these (mapping to 4676 DAVID IDs) were modulated by ≥1 E2s. d JUMPptm analyses identify linkage-specific ubiquitin modifications modulated by E2s. In particular, the knockdown of many E2s reduces K48-linked ubiquitination whereas this increases upon heat shock. Other linkage-specific modifications (K6, K11, K27, K29, K33, and K63) are affected by fewer E2s. Significant downregulation ( P < 0.05) of these modifications by E2 RNAi is displayed in bold fonts and shades of red whereas non-significant changes ( P > 0.05) are shown in gray. Supplementary Fig. reports the precise P values (one-way ANOVA). e Proteins that are regulated by E2 RNAi without significant mRNA changes are reported on the y -axis whereas the number of E2s regulating each protein subset is reported on the x -axis. Several GO categories are enriched among protein sets that are modulated by 5–9 E2s ( f ), 2–4 E2s ( g ), and by single E2s ( h ). Source data are provided in the Source data file.
Tmt 16plex Reagents A44522, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/tmt 16plex reagents a44522/product/Thermo Fisher
Average 90 stars, based on 1 article reviews
tmt 16plex reagents a44522 - by Bioz Stars, 2026-02
90/100 stars
  Buy from Supplier
tmt 1 lex/16plex reagent  (Thermo Fisher)
90
Thermo Fisher tmt 1 lex/16plex reagent
E2 knockdown in HEK293T cells was obtained via siRNAs for individual E2s or for groups of related E2s with high sequence homology (e.g. UBE2D1/2/3), which were targeted together by pooling E2-specific siRNAs. E2 siRNAs reduce (green) the mRNA ( a ) and protein ( b ) levels of the targeted E2s (boxed) specifically, i.e. without affecting other E2s, compared to non-targeting (NT) siRNAs. Significant ( P < 0.05; unpaired two-tailed t test) transcriptional changes are highlighted in bold red (downregulated) and blue fonts (upregulated). The extent of downregulation is displayed in green shades whereas non-significant changes are shown in gray. c Deep-coverage TMT mass spectrometry identifies the proteome subsets that are regulated by E2 RNAi. Compared to NT siRNAs, E2 RNAi leads to significant protein upregulation (blue) and downregulation (red) ( P < 0.05; Log 2 FC > 0.2 and <–0.2) that do not arise from corresponding changes in mRNA levels, defined by RNA-seq. This data represents 6 sets of <t>16-plex</t> TMTs of E2 siRNAs ( n = 3 biological replicates/group), each with its own control NT siRNAs ( n = 4 biological replicates). On average, each TMT set quantified 10700 proteins: 5132 of these (mapping to 4676 DAVID IDs) were modulated by ≥1 E2s. d JUMPptm analyses identify linkage-specific ubiquitin modifications modulated by E2s. In particular, the knockdown of many E2s reduces K48-linked ubiquitination whereas this increases upon heat shock. Other linkage-specific modifications (K6, K11, K27, K29, K33, and K63) are affected by fewer E2s. Significant downregulation ( P < 0.05) of these modifications by E2 RNAi is displayed in bold fonts and shades of red whereas non-significant changes ( P > 0.05) are shown in gray. Supplementary Fig. reports the precise P values (one-way ANOVA). e Proteins that are regulated by E2 RNAi without significant mRNA changes are reported on the y -axis whereas the number of E2s regulating each protein subset is reported on the x -axis. Several GO categories are enriched among protein sets that are modulated by 5–9 E2s ( f ), 2–4 E2s ( g ), and by single E2s ( h ). Source data are provided in the Source data file.
Tmt 1 Lex/16plex Reagent, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/tmt 1 lex/16plex reagent/product/Thermo Fisher
Average 90 stars, based on 1 article reviews
tmt 1 lex/16plex reagent - by Bioz Stars, 2026-02
90/100 stars
  Buy from Supplier
tmt 16-plex label reagent a44520  (Thermo Fisher)
90
Thermo Fisher tmt 16-plex label reagent a44520
E2 knockdown in HEK293T cells was obtained via siRNAs for individual E2s or for groups of related E2s with high sequence homology (e.g. UBE2D1/2/3), which were targeted together by pooling E2-specific siRNAs. E2 siRNAs reduce (green) the mRNA ( a ) and protein ( b ) levels of the targeted E2s (boxed) specifically, i.e. without affecting other E2s, compared to non-targeting (NT) siRNAs. Significant ( P < 0.05; unpaired two-tailed t test) transcriptional changes are highlighted in bold red (downregulated) and blue fonts (upregulated). The extent of downregulation is displayed in green shades whereas non-significant changes are shown in gray. c Deep-coverage TMT mass spectrometry identifies the proteome subsets that are regulated by E2 RNAi. Compared to NT siRNAs, E2 RNAi leads to significant protein upregulation (blue) and downregulation (red) ( P < 0.05; Log 2 FC > 0.2 and <–0.2) that do not arise from corresponding changes in mRNA levels, defined by RNA-seq. This data represents 6 sets of <t>16-plex</t> TMTs of E2 siRNAs ( n = 3 biological replicates/group), each with its own control NT siRNAs ( n = 4 biological replicates). On average, each TMT set quantified 10700 proteins: 5132 of these (mapping to 4676 DAVID IDs) were modulated by ≥1 E2s. d JUMPptm analyses identify linkage-specific ubiquitin modifications modulated by E2s. In particular, the knockdown of many E2s reduces K48-linked ubiquitination whereas this increases upon heat shock. Other linkage-specific modifications (K6, K11, K27, K29, K33, and K63) are affected by fewer E2s. Significant downregulation ( P < 0.05) of these modifications by E2 RNAi is displayed in bold fonts and shades of red whereas non-significant changes ( P > 0.05) are shown in gray. Supplementary Fig. reports the precise P values (one-way ANOVA). e Proteins that are regulated by E2 RNAi without significant mRNA changes are reported on the y -axis whereas the number of E2s regulating each protein subset is reported on the x -axis. Several GO categories are enriched among protein sets that are modulated by 5–9 E2s ( f ), 2–4 E2s ( g ), and by single E2s ( h ). Source data are provided in the Source data file.
Tmt 16 Plex Label Reagent A44520, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/tmt 16-plex label reagent a44520/product/Thermo Fisher
Average 90 stars, based on 1 article reviews
tmt 16-plex label reagent a44520 - by Bioz Stars, 2026-02
90/100 stars
  Buy from Supplier

Image Search Results


E2 knockdown in HEK293T cells was obtained via siRNAs for individual E2s or for groups of related E2s with high sequence homology (e.g. UBE2D1/2/3), which were targeted together by pooling E2-specific siRNAs. E2 siRNAs reduce (green) the mRNA ( a ) and protein ( b ) levels of the targeted E2s (boxed) specifically, i.e. without affecting other E2s, compared to non-targeting (NT) siRNAs. Significant ( P < 0.05; unpaired two-tailed t test) transcriptional changes are highlighted in bold red (downregulated) and blue fonts (upregulated). The extent of downregulation is displayed in green shades whereas non-significant changes are shown in gray. c Deep-coverage TMT mass spectrometry identifies the proteome subsets that are regulated by E2 RNAi. Compared to NT siRNAs, E2 RNAi leads to significant protein upregulation (blue) and downregulation (red) ( P < 0.05; Log 2 FC > 0.2 and <–0.2) that do not arise from corresponding changes in mRNA levels, defined by RNA-seq. This data represents 6 sets of 16-plex TMTs of E2 siRNAs ( n = 3 biological replicates/group), each with its own control NT siRNAs ( n = 4 biological replicates). On average, each TMT set quantified 10700 proteins: 5132 of these (mapping to 4676 DAVID IDs) were modulated by ≥1 E2s. d JUMPptm analyses identify linkage-specific ubiquitin modifications modulated by E2s. In particular, the knockdown of many E2s reduces K48-linked ubiquitination whereas this increases upon heat shock. Other linkage-specific modifications (K6, K11, K27, K29, K33, and K63) are affected by fewer E2s. Significant downregulation ( P < 0.05) of these modifications by E2 RNAi is displayed in bold fonts and shades of red whereas non-significant changes ( P > 0.05) are shown in gray. Supplementary Fig. reports the precise P values (one-way ANOVA). e Proteins that are regulated by E2 RNAi without significant mRNA changes are reported on the y -axis whereas the number of E2s regulating each protein subset is reported on the x -axis. Several GO categories are enriched among protein sets that are modulated by 5–9 E2s ( f ), 2–4 E2s ( g ), and by single E2s ( h ). Source data are provided in the Source data file.

Journal: Nature Communications

Article Title: An adaptive stress response that confers cellular resilience to decreased ubiquitination

doi: 10.1038/s41467-023-43262-7

Figure Lengend Snippet: E2 knockdown in HEK293T cells was obtained via siRNAs for individual E2s or for groups of related E2s with high sequence homology (e.g. UBE2D1/2/3), which were targeted together by pooling E2-specific siRNAs. E2 siRNAs reduce (green) the mRNA ( a ) and protein ( b ) levels of the targeted E2s (boxed) specifically, i.e. without affecting other E2s, compared to non-targeting (NT) siRNAs. Significant ( P < 0.05; unpaired two-tailed t test) transcriptional changes are highlighted in bold red (downregulated) and blue fonts (upregulated). The extent of downregulation is displayed in green shades whereas non-significant changes are shown in gray. c Deep-coverage TMT mass spectrometry identifies the proteome subsets that are regulated by E2 RNAi. Compared to NT siRNAs, E2 RNAi leads to significant protein upregulation (blue) and downregulation (red) ( P < 0.05; Log 2 FC > 0.2 and <–0.2) that do not arise from corresponding changes in mRNA levels, defined by RNA-seq. This data represents 6 sets of 16-plex TMTs of E2 siRNAs ( n = 3 biological replicates/group), each with its own control NT siRNAs ( n = 4 biological replicates). On average, each TMT set quantified 10700 proteins: 5132 of these (mapping to 4676 DAVID IDs) were modulated by ≥1 E2s. d JUMPptm analyses identify linkage-specific ubiquitin modifications modulated by E2s. In particular, the knockdown of many E2s reduces K48-linked ubiquitination whereas this increases upon heat shock. Other linkage-specific modifications (K6, K11, K27, K29, K33, and K63) are affected by fewer E2s. Significant downregulation ( P < 0.05) of these modifications by E2 RNAi is displayed in bold fonts and shades of red whereas non-significant changes ( P > 0.05) are shown in gray. Supplementary Fig. reports the precise P values (one-way ANOVA). e Proteins that are regulated by E2 RNAi without significant mRNA changes are reported on the y -axis whereas the number of E2s regulating each protein subset is reported on the x -axis. Several GO categories are enriched among protein sets that are modulated by 5–9 E2s ( f ), 2–4 E2s ( g ), and by single E2s ( h ). Source data are provided in the Source data file.

Article Snippet: The purified peptides were resuspended in 50 mM HEPES (pH 8.5) and labeled with 16-plex Tandem Mass Tag (TMT) reagents (ThermoScientific) following the manufacturer’s recommendations.

Techniques: Sequencing, Two Tailed Test, Mass Spectrometry, RNA Sequencing Assay